Dr. Neeta Shrivastava

Total Experience 20 years

Educational Qualification : Ph.D

Research Area: Plant Tissue Culture, Molecular Biology, Genetic Engineering, Phytochemistry, Phytopharmacology


General information
Designation: Assistant Director
Office Phone 07927439375
Email neetas@perdcentre.com

Brief about you

Dr. Neeta completed her basic education in Plant Sciences from Dr. HS Gour University Sagar, Sagar and The MS University of Baroda Vadodara. She retrained herself in Phytochemistry, phytopharmacology and Biotechnology during her profession. She has 65 publication to her credit which includes research article, review article, book chapter monographs, and general article. She has guided more then 30 masters students for dissertation work, and 9 student for doctoral work. She has won Rajanibhai Patel PharmaInnova award for the year 2014-15 in pharmacognosy category.

Current Activities :

Working on Metabolic engineering, DNA Barcoding, Genetic engineering, Role of cell competition in Cancer and Nephrotoxicity

Experience
Research 20 years
Teaching 10 years
Industry -
Contact
Website www.perdcentre.com
Membership IBSC Nirma University, GSBTM
Specialization
Specialization Areas: Cell biology, Molecular biology, Genotoxicity, Immunopharmacology, Microbial Genetics
Subject Taught UG: -
Subject Taught PG: -
Sr. No. Position Duration Organization
Assistant Director Since April 2011 B. V. Patel PERD Centre, Ahmedabad
*Project Director July 2010- November 2014 National institute of Pharmaceutical education and research (NIPER) , Ahmedabad (under ministry of Chemicals & Fertilizers)
Scientist C March 2008 – March 2011 B. V. Patel PERD Centre, Ahmedabad
Scientist B June 2003 to February 2008 B. V. Patel PERD Centre, Ahmedabad
Scientist A Jan. 1999 to May 2003 B. V. Patel PERD Centre, Ahmedabad
Research Assistant Dec. 1997 to Dec. 1998 B. V. Patel PERD Centre, Ahmedabad
Post Doctoral Fellow Sept. 1996-Dec. 1997 B. V. Patel PERD Centre, Ahmedabad

#An additional charge of Project Director-NIPER-Ahmedabad from July 2010- November 2014

A. International Journals

Sr. No. Title ISSN/ISBN No Journal/ Publisher Volume Year
1. Genetic diversity in chemically diverse accessions of Bacopamonnieri,   Journal of Planner Chromatography-Modern TLC (in Press)  
2. Renaissance in phytomedicines- promising implications of NGS technologies.   Accepted in Planta, DOI 10.1007/s00425-016-2492-8  
3. Internal transcribed spacer guided multiplex PCR for species identification of Convolvulusprostratus and Evolvulusalsinoides traded as "Shankhpushpi"   Acta Pharmaceutica Sinica B 6(3):253-258 2016
4. Cell Competition: Roles And importance as central phenomenon. Critical ReviewsTM in   Eukaryotic Gene Expression, 25 (4), 307-314 2015
5. In Silico Screening of Alleged miRNAS Associated with Cell Competition: an Emerging Cellular Event   Cancer. Cellular and Molecular Biology letters. Nov. 5, DOI:10.1515/cmble-2015-0046. 2015
6. The shRNA mediated silencing of VEGF-C illustrates its role in proliferation, chemosensitisation, tumor colonization and anchorage independence.   BioTechnologia 2015, 96 (3) pp. 225-233 2015
7. Strain specific agrobacterium-mediated genetic transformation in Bacopamonnieri.,   Journal of Genetic Engineering and Biotechnology 12, 89-94 2014
8. Hairy root culture: A suitable biological system for studying sedcondary metabolic pathways in plants.   Engineering in Life Sciences, 13,62-75 2013
9. Methyl jasmonate mediates upregulation of bacoside A production in shoot cultures of Bacopamonnieri.   Biotechnology Letters 35 (7), 1121-1125, DOI :10.1007/s10529-013-1178-6 2013
10. A combination approach for rapid and high yielding purification of bacterial minicell.   Journal of Microbiological Methods 92, 340-343 2013
11. Ecogeographicalphytochemistry of Adhatodavasica Nees. In relation to quantitative variation of alkaloids.   Journal of Planar Chromatography- Modern TLC, 24, 406-411, (Impact factor, 2010-1.247). 2011
12. Development of sex-linked AFLP markers in Simmondsia chinensis. Plant Breeding,     2010 (doi: 10.111/j.439-0523.2009.01749.x) (Impact Factor, 2009- 1,027).  
13. Microscale procedure for analysis of Andrographolide in Andrographispaniculata leaves.   Journal of Planar Chromatography 23 53-58, (Impact factor, 2010-1.247). 2010
14. Production of heterologous proteins in plants: Strategies for optimal expression.   Biotechnology Advances 28, 427-435,. (Journal Impact Factor, 2009 - 8.25) 2010
15. Andrographolide: Anew plant derived antineoplastic entity on horizon.   eCAM (doi: 10.1093/ecam/nep135) (Impact Factor,2009 - 2.0640 2009
16. RNA interference: An emerging generation of biologicals.   Biotechnology Journal 3, 339-353, (Impact Factor, 2009 - 2.1). 2008
17. Phytochemical and preclinical screening of aseptically produced herbal raw material: Bacopamonnieri.   Medicinal and Aromatic Plant Science and Biotechnology 2, 123-127, 2008
18. Endometrial membrane response in Musmusculus during implantation by Vitexnegundo Linn   Animal Reproduction 4, 46-50, (Impact factor, 2010-1.721). 2007
19. Sustainable production of a therapeutically important tree (Holarrhenaantidysenterica) of India.   Medicinal and Aromatic Plant Science and Biotechnology 1, 151-154, 2007
20. Plant genomic DNA isolation: An art or a science.   Biotechnology Journal 2, 386-392, (Impact Factor, 2009 - 2.1). 2007
21. Clerodendrum and health: An overview..   Medicinal and Aromatic Plant Sciences and Biotechnology 1, 142-150, 2007
22. Anti-hyperlipidemic effect of Carica papaya L. in Sprague-Dawley rats.   Nigerian Journal of Natural Products and Medicine 69-72, 10, 2006
23. Ex situ conservation method for Clerodendruminerme: a medicinal plant of India.   African Journal of Biotechnology 5, 415-418, (Impact factor, 2010-0.573). 2006
24. Anti ulcer activity of Adhatodavasica Nees.   Journal of Herbal Pharmacotherapy 6, 43-50, (Impact factor, 2010-2.208). 2006
25. Blastocyte implantation failure due to 'non receptive endometrium': endometrial alterations by Hibiscusrosasinensis leaf extract.   Contraception). 71, 227-230, (Impact factor, 2010-2.511 2005
26. Sperm membrane modulation by Sapindusmukorossi during sperm maturation. (* This paper was nominated for Royan Society Inter National Award)   Asian Journal of Andrology 4, 232-235, (Impact factor, 2010-3.141). 2002
27. Evaluation of antioxidant properties of root bark of Hemidesmusindicus R. Br. (Anantmul).   Phytomedicine 9, 153-160, (Impact factor, 2008-2.3). 2002
28. HPTLC aided phytochemical fingerprint analysis as a tool for evaluation of herbal drugs - A case study of Ushaq (Ammoniacum gum).   Journal of Planar Chromatography 14, 34-41, (Impact factor, 2010-1.247). 2001
29. HPTLC method for the estimation of alkaloids of Cinchona stem bark and its marketed formulations.   Planta Medica 67, 294-296, (Impact factor, 2010- 2.369). 2001
30. A sensitive HPTLC method for the estimation of diospyrin, a tumor; inhibitory agent from stem bark ofDiospyrosmontanaRoxb.   Journal of Chromatography B 744, 257-262, (Impact factor, 2010- 3.00). 2000
31. A rapid method for isolation of androgapholide from AndrographispaniculataNees (Kalmegh).   Pharmaceutical Biology 38, 204-209, (Impact factor, 2009-0.672). 2000
32. Multiple shoot regeneration and tissue culture studies on Bacopamonnieri (L.)   Pennell. Plant Cell Reports 18, 919-923, (Impact factor, 2009- 2.279). 1999

B. National Journals

Sr. No. Title ISSN/ISBN No Journal/Publisher Volume Year
1. DNA Based Simultaneous Identification of Three Terminalia Species Targeting Adulteration,   Pharmacognosy Magzine (in Press)  
2. "Homogenous PCR of Heterogeneous DNA from Phenolic Rich Barks of Terminalia Species for DNA Based Adulteration Detection"   Proceedings of National Academy of Sciences, India section B, Biological Sciences (In Press), doi10.1007/s40011-015-0576-z  
3. Study on antibacterial potential of Physalisminima Linn.   Indian Journal of Pharmaceutical Sciences, 73 (1) 111-115, 2011
4. N. Antioxidant and cytotoxic potential of Physalis minima and Solanumnigrum.   Indian Drugs, 47(4), 2010
5. Phytochemical evaluation and radical scavenging activity of three members from family Asteraceae.).   Indian Drugs, 44 (Impact Factor, 2009 -0.028 2007
6. Effect of sucrose concentrations on regenerative potential of Bacopamonnieri (L.) Pennell.   Indian Drugs 44, 474-477, (Impact Factor, 2009 -0.028). 2007
7. Analgesic and anti-inflammatory activity of hydroalcoholic extract of MorusalbaLinn.   Indian Drugs (Accepted for publication) (Impact Factor, 2009 -0.028).  
8. Antibacterial activity of petroleum ether extract of fennel and cumin.   Advances in Biological Sciences   2006
9. Biosynthetic potential of in vitro grown callus cells of Cassia senna L. varsenna.   Current Science 90, 1472-1473, (Impact Factor, 2010-0.782). 2006
10. Antiulcer activity of methanolic extract of Sapindusmukorossi.   Indian Drugs 42, (Impact Factor, 2009 -0.028). 2005
11. Wound healing activity of Tridaxprocambance.,   Advances in Pharmacology Toxicology   2005
12. Antiulcer activity of methanolic extract of Ecliptaalba.   Indian Journal of Pharmaceutical Sciences, 165-168, (Impact Factor, 2009, -0.044). 2005
13. A study of antimicrobial activity of Elephantopusscaber.   Indian Journal of Pharmacology, 126-127, 2005
14. Antimicrobial activity of Ecliptaalba.   Indian Drugs, 42, 133-135 (Impact Factor, 2009, -0.028). 2005
15. Simple spectrophotometric method for the estimation of total alkaloids from seeds of Lupinusalbus L.   Indian Drugs 39, 494-496, (Impact Factor, 2009, -0.028). 2002
16. Simultaneous determination of four alkaloids of Cinchona officinalis from the stem bark and formulations containing Cinchona by HPLC method.   Indian Drugs 38, (Impact Factor, 2009, -0.028). 2001
17. Spectrophotometric estimation of total alkaloids from Cinchona officinalis stem bark and marketed formulations containing Cinchona.   Indian Journal of Pharmaceutical Sciences, 63, 76-78, (Impact Factor, 2009, -0.044). 2001
18. Punarnavine' profile in the regenerated roots of BoerhaaviadiffusaL from leaf segments.   Current Science 68, 653-656, (Impact Factor,2010-0.782). 1995

C. Book Chapters

Sr. No. Title
1. Poojadevi Sharma, Sonal Sharma, Sheetal Yadav, Anshu Srivastava, Indira Purohit, Neeta Shrivastava. Plant derived bioactive molecules: culture vessels to bioreactor. In Production of Biomass and Bioactive compounds using bioreactor technology, Eds: K. Y. Paek et al. Springer Science and Business Media, Dordrecht, 2014, pp 47-60.
2. Anshu Srivastava, Neeta Shrivastava. Direct shoot regeneration of Cassia sennafor sustainable production of anthraquinone glycoside - sennoside, Chapter 29, in 'Applications of Plant Biotechnology: In Vitro Propagation, Plant Transformation and Secondary Metabolite Production', Eds: Ashwani Kumar and Sudhir K Sopory, 2010, IK International Publishing House, New Delhi, (ISBN: 9789380026930).
3. Astha Varma and Neeta Shrivastava.The role of plant genomics in biotechnology. In: Encyclopedia of Life Support Systems (EOLSS), Ed. Doelle HW, in Encyclopedia of Life Support Systems (EOLSS), Developed under the Auspices of the UNESCO, Eolss Publishers, Paris, France [http://www.eolss.net],2008.ISBN:978-1-84826-258-8 (e book)
4. Neeta Shrivastava, Niranjan Kanaki, Ravishankar MN, M. Rajani. Standardization of Til-e-Mubahhi: A Unani formulation. In: Traditional System of Medicine, Eds. Abdin MZ and Abrol YP, Narosa Publishing House, New Delhi, 2006.
5. Avani Kothari, Harish Padh, Neeta Shrivastava. In vitro plantlet regeneration of medicinally important plant Clerodendruminermi. In: Biotechnology for Better Future, SAC Publication, Mangalore, 2004, 138-142.
6. Anshu Shrivastava, Neeta Shrivastava, Niranjan Kanaki, M. Rajani, Harish Padh. Axillary bud multiplication and phytochemical evaluation of Kurchi: An Indian medicinal plant. In: Biotechnology for Better Future, SAC Publication, Mangalore, 2004, 106-110.
7. M. Rajani, Neeta Shrivastava, Ravishanker MN. Brahmi (Bacopa monieri (L.) Pennell.) - A medhya rasaayana drug of Ayuveda. In: Biotechnology of Medicinal Plants Vitalizer and Therapeutics, Ed. Ramavat KG, Science Publishers, Inc. Plymouth UK, 2004.

D. General Articles

Sr. No. Title
1 An emerging class of therapeutics. Souvenir of 5th International Symposium on 'Innovations in Pharmaceutical Sciences and Technology', Mumbai. February 1-3, 2003. pp. 75-80.
2 Monoclonal antibodies (mAb) as therapeutics. Proceedings of Andhra Pradesh Academy of Sciences 8, 2004.
3 Biotechnology derived therapeutics. Souvenir of National seminar on '50 Years of DNA Double Helix Retrospect and Prospects', Rajkot, 2004.
4 Biotechnology - An age old modern technology.. Souvenir of National Seminar on 'Recent Development in Chemical Technologies', Ahmedabad. October 7, 2006. pp. 31-40

E. Awards

Sr. No. Title
Award for Best Research Guide (M. Pharm Thesis 2014-15) in the subject Pharmacognosy and Phytochemistry
Best poster Award (rank 3) in National Seminar on ‘Herbal Drugs Research: Opportunities and Challenges’ November 5-7, 2014, Ahmedabad.
Best poster award in 94th annual meeting of plant tissue culture
Best Poster award in UGC –DSA symposium at MS University
Award for Best Research Guide (M. Pharm Thesis 2014-15) in the subject Pharmacognosy and Phytochemistry

F. Patents

Sr. No. Title
The patent is entitled as “Multiplex PCR based single tube assay for unequivocal identification and detection of Convolvulus microphyllus and its adulterant/allied species Evolvulusalsinoides”. Invented process is protected from 22 June 2014. Application number is 2017/MUM/2014

G. Sequence submission to NCBI : 7 (Publication)

Sr. No. Plant Species Name Gene Accession Number in NCBI Name of the Sequenced Region Submitted By
1 Terminaliabellerica(Common Name- Baheda) KC602394 ITS 1 and 2 Sonal Sharma and Dr. Neeta Shrivastava
2 Terminaliachebula(Common Name- Harad) KC750922
3 Convolvulus microphyllus(Common Name- SafedShankhpushpi) KC688314
4 Evolvulus alsinoides(Common Name- Kali Shankhpushpi) KC688313
5 Terminaliaarjuna(Common Name- Arjun) KF925432 ITS 1 and 2 Sonal Sharma, Neeta Shrivastava, C. G Joshi,
Prakash S.
6 Saraca asoca(Common Name- Ashok) KF925433 ITS 1 Sonal Sharma, Poojadevi Sharma, SheetalYadav, Dr Anshu Shrivastava, Neeta Shrivastava, Dr. C. G Joshi, Prakash S. D.
7 Terminalia tomemtosa KT187391 ITS Sonal Sharma, Neeta Shrivastava
Qualification Specialization University Passing Year
Ph.D. Plant Tissue Culture The MS University of Baroda, Baroda 1995

Training Received

  • Hands on Training workshop on ‘Flow cytometry and Sorting’ organized by BD – NCCS at NCCS Pune, June 22-24, 2015
  • Faculty Development Program on DNA Barcode: organized by Gujarat State Biotechnology Mission, May 15-21, 2013
  • Human Embryonic Cells: Differentiation and Genetic Manipuation, January18-23.2010. Stem Cell Biology Dept., NIRRH, Parel Mumbai, sponsored by ICMR
  • Plant Genomics 2006 : Application of DNA Based Molecular Markers for Crop Improvement’ March 16 – 29, 2006, organized by National Centre for Plant Genome Research, New Delhi, 110067, India
  • Plant Genetic Engineering, December 7-21, 2004, Organised by Indian Academy of Sciences and Madurai Kamraj University at Madurai, Tamil Nadu, India.
  • Development of Standard Operating Procedure, August 11-17, 2003, Organized by Ministry of Health and Family welfare, Dept. of Indian System of Medicine and Homeopathy at National Botanical Research Institute, Lucknow, UP, India
  • Standardization and Quality Control of ISM drugs, December 3-17, 1998, Organized by, Organized by Ministry of Health and Family welfare, Dept. of Indian System of Medicine and Homeopathy at Pharmacopoeia Laboratory for Indian Medicine at Ghaziabad, UP
Activity Number
Workshop /CE programme coordinator 20
Member of organizing committee in International symposium 4

A. Government Projects

S. No. Fund Type Agency Start Date Duration
1. Govt. of India DBT-BIRAC    
2. Govt. of India Dept. of Biotechnology,    
3. Govt. of Gujarat, Gujarat State Biotechnology Mission (GSBTM), 2011 3 years
4. Govt. of India Department of Biotechnology, Ministry of Science and Technology, 2008 3 years
5. Govt. of India Department of Biotechnology, Ministry of Science and Technology, 2002 3 years
6. Govt. of India Indian Council of Medical Research 2002 6 years
7. Govt. of Gujarat Gujarat Council of Science & Technology (GUJCOST), 2002 2 years

B. Industry Projects

Sr. No. Title
1. Many industry sponsored and in house project related to phytochemical analysis and standardization

C. In house projects

Sr. No. Title
1. Production of therapeutic protein (EPO) in plants
2. Metabolic engineering in Plants
3. Inhibition of molecular target in breast cancer using RNAi
4. Cell competition
5. Nephrotoxicity